Has anybody got previous experience using Matrigel?

More Shaima Jabbar's questions See All

Which plagiarism software is suitable to use?

Hello I am looking for plagiarism software that able to check published pape. In other words can exclude the publication yea. I appreciate your suggestion

03 April 2019 7,211 37 View

What is the difference between Thomson Reuters and Impact factor?

Hello everyone I am totally confused between the terms of journals have impact factor, journals have Scopus index number and journals have Thomson Reuters index number. Could anyone explain for...

02 March 2018 6,948 6 View

Any tips with antibodies similarity?

Hello everyone I am looking for CD24 Ab for rat. unfortunately, the most antibodies are available for human and mouse. Does anybody know how to look for antibodies similarity between rat and...

05 June 2016 3,153 2 View

Does anyone have background in the RNA seq bioinformatics?

Hello everyone I am looking for open courses for RNA seq bioinformatics. Does anyone have any idea about good website. Thanks for your time Shaima Jabbar

08 September 2015 5,003 2 View

Did anyone do primary cell line from spleen?

I need to prepare primary cell from rat spleen. If anybody have done that before, please advise me which method is good for that kind of culture.

01 February 2015 6,741 3 View

How do I eliminate a fungal contamination in my cancer cell culture?

Greeting everyone I am doing primary culture of cancer cells, but I am facing fungus contamination. Does anyone have any idea what should I do. I really appreciate your help and time. Thanks

31 December 2014 7,732 2 View

Does anyone know a suitable kit to measure cells proliferation?

Greeting everyone, I have cancer cell line, and I need to measure cells proliferation. Does anyone have experience with this assay? Thanks shaima Jabbar

10 November 2014 1,733 13 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View