Does anyone have background in the RNA seq bioinformatics?

More Shaima Jabbar's questions See All

Which plagiarism software is suitable to use?

Hello I am looking for plagiarism software that able to check published pape. In other words can exclude the publication yea. I appreciate your suggestion

03 April 2019 7,211 37 View

What is the difference between Thomson Reuters and Impact factor?

Hello everyone I am totally confused between the terms of journals have impact factor, journals have Scopus index number and journals have Thomson Reuters index number. Could anyone explain for...

02 March 2018 6,948 6 View

Any tips with antibodies similarity?

Hello everyone I am looking for CD24 Ab for rat. unfortunately, the most antibodies are available for human and mouse. Does anybody know how to look for antibodies similarity between rat and...

05 June 2016 3,153 2 View

Did anyone do primary cell line from spleen?

I need to prepare primary cell from rat spleen. If anybody have done that before, please advise me which method is good for that kind of culture.

01 February 2015 6,741 3 View

Has anybody got previous experience using Matrigel?

I am planing to subcutaneously implant cells into mice using Matrigel. Does anyone have any guidance regarding the concentration on Matrigel to be used with cells, or indeed any tips on using...

31 December 2014 946 3 View

How do I eliminate a fungal contamination in my cancer cell culture?

Greeting everyone I am doing primary culture of cancer cells, but I am facing fungus contamination. Does anyone have any idea what should I do. I really appreciate your help and time. Thanks

31 December 2014 7,732 2 View

Does anyone know a suitable kit to measure cells proliferation?

Greeting everyone, I have cancer cell line, and I need to measure cells proliferation. Does anyone have experience with this assay? Thanks shaima Jabbar

10 November 2014 1,733 13 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Is this website real?

Is this website real? https://isar.org.in/event/registration.php?id=2434532

08 August 2024 484 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

U you think We need a website software of Blackbody radiation law expert software?

A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index

07 August 2024 1,706 0 View

Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Rokib Hasan

I think you can learn a lot things from this book titled "RNA Sequence, Structure, and Function: Computational and Bioinformatic Methods" by Gorodkin, Jan, Ruzzo, Walter L. (Eds.).

As far open course I found very small things... please find the following links, which will help you certainly...

http://www.bioinformatics.babraham.ac.uk/training.html#rnaseq

http://www.rna-seqblog.com/list-of-rna-seq-bioinformatics-tools-2/

http://bioinformatics.ca/workshops/2013/informatics-rna-sequence-analysis-2013

I personally also think that you will get a lot of info from here too...

https://en.wikipedia.org/wiki/List_of_RNA-Seq_bioinformatics_tools

Best of luck!

Shaima Jabbar

Thank you so much