I assume this is in the mouse? Just perfuse the BM with PBS or RPMI and culture all the cells, then use magnetic bead selection to pick out the macrophages.

Do you want to isolate BM macrophage progenitors or want to generate BM derived macrophages?

We have a protocol for Bone marrow macrophages from mice. If you need it, send your email address on Researchgate. Cheers

Dear Dr. Kurakula,

Many thanks for you help and my email is [email protected]

Best,

Mostafa

Dear Menon, I want to isolate BM macrophage progenitors from mice. Thanks

Hi there,

We have a simple protocol on our lab's website involving bone marrow isolation of macrophages. It used by a lot of labs worldwide.

www.bowdish.ca under protocols