Dear Mehdi Babaei

Please find your answer in the attached pdf file of a related article.

Dear  kazem ghassemi

Many thanks ,it is good

Hi Maehdi, 

I think you question is technical. You are asking if it is possible to use the microplate reader to quantify chlorophyll a, chlorophyll b and carotenoids based on the formula given for 1 cm pathlengh cuvettes (for example, Wellburn method, Vol. 144, No. 3, 1994, p. 307-313)?? 

The answer is YES, and you need to use a constant factor to get the final corrected data equivalent to what you will get in a 1 cm pathlength cuvette. This depends on the amount of liquid used (well length, lightpathlength). In my case, the plate reader and plate I use, I need to multiply the data with 3.5 constant factor when I use 100 ul extracts per well. but this factor will change if I use 200 or 250 ul.

Hope this helps.

Thanks

Sushanta

hi dr kumar saha

Thank you very much for the detailed explanation