I never have had issues in the past and recently with the new red kits I have been having the worst results with my extractions.
Yes. Would love to figure out how to optimize/workaround.
The steps recommended here and following with an EtOH precipitation worked for me if anyone is still looking for the answer for this. https://www.biocompare.com/Product-Reviews/130689-mirVana-microRNA-Isolation-Kit-from-Life-Technologies/
after effects of civid 19 pandemic.
25 November 2023 6,705 0 View
What is the perfect philosophical underpinning for a sequential explanatory approach?
10 October 2019 7,724 4 View
Actually the phases are matched aptly but while doing the refinement the it gives a increased Rw. I want to cut it down to a minimum value through essential parameter adjustment.
07 May 2019 8,276 1 View
I have 32 samples (16 +16 of two conditions) of RNASeq data and after differential expression analysis using DESeq2, I used WGCNA to make gene modules. To visualize the networks to which the...
01 August 2018 7,981 2 View
I would like to find out, what is the frequency of concurrent infections with RSV and Influenza (A/B virus)?
17 April 2016 4,412 3 View
I would like to include in my prospectus a questionnaire for health providers regarding the psychosocial treatment for type-2 diabetes American Indians?
23 September 2015 6,313 4 View
I am trying to assist a researcher who wants to confirm a protein ID she has from a western blot. She'd like to run just the western band, and rather than the full in-gel she'd like to run after...
13 September 2015 6,492 1 View
The earthquake caused a 50-meter seaward tidal shift. There are exposed brain corals and branching coral recruits within the sanctuary that started bleaching
17 June 2015 7,782 6 View
Hi, can someone please share a link about the type of hardware and software vulnerabilities that leads to an attack please?
21 October 2014 5,929 12 View
Hi, I would like some papers / articles about the most common malware attacks and attacks on a computer that can infect other computers please!
17 October 2014 3,457 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
how can I extract my bibliography from researchgate ?
28 July 2024 6,737 1 View
Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.
26 July 2024 1,469 1 View