Hi Muthu,

Generally, the fungal assisted flocculation could be performed by two techniques. The first technique is the co-cultivation of microalgae with fungal pellets. This technique needs two steps of pelletization of fungal cells and co-cultivation with microalgae which required at least 72 and 48 h, respectively. The second technique is the co-pelletization in which the fungi grow and simultaneously flocculate microalgal cells in their pellets. This technique could reduce the operating steps and increase the overall productivity. In this paper, the first technique was used for our study. The microalgae was harvested within 10 min by 94%. The fungal Trichoderma is oleaginous fungi strain (>20% lipid content) which was used for this purpose. Therefore, after harvesting the biomass and lipid yields of microalgae-fungi pellets could improve. However, it is not possible to separate the microalgae from microalgae-fungi pellets due to entrapment in the pellets and attachment with exopolysaccharides, possibly secreted by the fungi.

I am working PhD project under supervision of Prof.Benjamas Cheirsilp, please contact her if you need to collaborate on your project.

If you have any question, please feel free to contact me.

Best regards,

Sirasit Srinuanpan

Thanks for your response Dr. Sirasit Srinupan. I will be grateful if you could forward my concern to your mentor. Since you are the scholar directly involved int his study, I request you to be a moderator of this collaboration.

Thanks.

Please contact her directly to discuss via her email: [email protected]

Also, my personal email is [email protected]

Best,

Sith

Hi Arumugam

I think this reference very useful = Exploring Pellet Forming Filamentous Fungi

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwiOrbS8sOjgAhUR-qQKHWkmANQQFjACegQIBRAB&url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F262771749_Exploring_Pellet_Forming_Filamentous_Fungi_as_Tool_for_Harvesting_Non-flocculating_Unicellular_Microalgae&usg=AOvVaw0A6_WTFo-pw_oZHFMXeh8c

Ref. = The mechanism of microalgal adhesion to fungal pellets

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=2ahUKEwiOrbS8sOjgAhUR-qQKHWkmANQQFjADegQIBxAC&url=https%3A%2F%2Fstud.epsilon.slu.se%2F8657%2F11%2Folsson_n_151208.pdf&usg=AOvVaw3VwnlBVzrnnSPWVSfRaFbZ

Ref.2 = A rapid method for harvesting and immobilization of oleaginous

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=2ahUKEwiOrbS8sOjgAhUR-qQKHWkmANQQFjAEegQIBhAB&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpubmed%2F30095310&usg=AOvVaw2bbuKfe5XcAb7mQw898k90

Ref. = Review of Microalgae Harvesting via Co-Pelletization

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&cad=rja&uact=8&ved=2ahUKEwiOrbS8sOjgAhUR-qQKHWkmANQQFjAHegQIAhAC&url=https%3A%2F%2Fwww.mdpi.com%2F1996-1073%2F6%2F11%2F5921%2Fpdf&usg=AOvVaw0-YVpwpGeFQWWrasuvO32X

Ref. = Harvesting of Chlorella sp. by Co-cultivation with Some Filamentous

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=2ahUKEwiOrbS8sOjgAhUR-qQKHWkmANQQFjAIegQIAxAC&url=http%3A%2F%2Fmjs.uomustansiriyah.edu.iq%2Fojs1%2Findex.php%2FMJS%2Farticle%2Fdownload%2F497%2Fpdf&usg=AOvVaw3Ia9iPHdiSnZU0oY7Has-H

Ref. = A rapid method for harvesting and immobilization

https://www.semanticscholar.org/paper/A-rapid-method-for-harvesting-and-immobilization-of-Srinuanpan-Chawpraknoi/8cd1d933f3588978b4a95f9dae8ed9249226a4fa

Ref.3 = Study of pellet formation of filamentous fungi Rhizopus oryzae

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=22&cad=rja&uact=8&ved=2ahUKEwiqkPHbs-jgAhVSIMUKHfk1BPg4ChAWMAt6BAgIEAE&url=https%3A%2F%2Fonlinelibrary.wiley.com%2Fdoi%2Fabs%2F10.1002%2Fbit.21531&usg=AOvVaw0JVxKAZ0w_rAUBg1-jkRcP

Ref. = Flocculation mechanism of Aspergillus niger on harvesting

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=21&cad=rja&uact=8&ved=2ahUKEwiqkPHbs-jgAhVSIMUKHfk1BPg4ChAWMAp6BAgCEAE&url=https%3A%2F%2Fscinapse.io%2Fpapers%2F2720989364&usg=AOvVaw32XznX-iAA2QOLojCetgXl

Ref. = The Efficacy of Ethyl Acetate Extract of Trichoderma Culture

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=23&cad=rja&uact=8&ved=2ahUKEwiqkPHbs-jgAhVSIMUKHfk1BPg4ChAWMAx6BAgFEAE&url=https%3A%2F%2Fwww.tci-thaijo.org%2Findex.php%2Fcast%2Farticle%2Fview%2F25931%2F21981&usg=AOvVaw1fDUawHECl2QrTCSfF2_0D

Dear Prof. Laith Al-Ani,

Many thanks for your response. Certainly i will refer the suggested references.

Best regards,

arumugam