B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Mixed ANOVA independent variables understanding in SPSS?

I have an experiment where 10 groups of 3 judges are asked to evaluate a list of pictures in 2 evaluation conditions. The pictures were made in 2 between-subjects conditions (A,B) and each group...

20 December 2020 7,697 3 View

How to modelling the concrete rigid slabs in a masonry building ?

I am working on developing an accurate 3D model. When I defined the rigid slab the analyses aborted for the static step. In the Message file appeared this: ***NOTE: THE SOLUTION APPEARS TO BE...

22 July 2020 3,431 2 View

Any suggestion on how to solve problems on DNA gel electrophoresis?

Please find attached a photo of the problem; After a simple Agarose gel electrophoresis of DNA with EtBr i find the gel "divided" in two parts. Analizing at the gel at 259nm it seems that the...

28 April 2020 2,327 7 View

What kind of pharmacological / biological behavior is this?

14 April 2020 9,913 10 View

Can I use "all rights reserved" data for scientific use ?

Hi everyone, I am wondering if I can use "all rights reserved" resources (mainly texts) extracted from the web for testing my models and then (eventually) pubblish a paper. Notice that No data...

29 March 2020 5,026 4 View

Is there exist a tool which extract lexical and synctactical features from a raw text?

Is there exist a tool which extract lexical (e.g average word length, frequency of words) and synctactical features (e.g appositions) from a text?

09 March 2020 4,867 2 View

No title

05 February 2020 4,691 4 View

How can we conceptualise and explore the relationship between nationalism and climate change?

I have searched some of the top ranking nationalism studies journals (Nations and nationalism, SEN -Studies of ethnicity and nationalism, Nationalism and ethnic politics, Ethnicities,...

28 August 2019 1,344 13 View

How can I prepare a food simulant for the evaluation of thermal denaturation of proteins?

I am interested in understanding the effect of thermal treatments on proteins denaturation into food simulant. Actually, I have already performed tests onto acqueous solutions of different kind of...

26 June 2019 2,525 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View