Dear Researchers,

I have a batch of slides (mouse muscle cryosections) from a pretty precious origin, and I try to make the best use of every slide.

I made a staining with an antibody (Alexa 488) and with a-bungarotoxin (Alexa 594 conjugate). Anyway, antibody staining looked rather unspecific, so I had meaningful signals only from the 594 channel (and DAPI).

I am curious if I can do classic H&E staining with my slides, simultaneously retaining the a-bungarotoxin signal on the 594 channel?

Best,

Michail

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