Actually i used plant extract to determine whether there is antibacterial activity or not.
i think can use because is it just blank disc. can anyone give their opinion too?
Yes, if you have no other option, you can use expired blank sterilized discs for plant extract in an antimicrobial susceptibility test (AST). However, it is important to note that doing so is not recommended due to potential concerns regarding accuracy and reliability of the results.
If the disk is truly blank and you are adding extract to it, then it should be fine even past the expiration date. I think your only major concern is to ensure the disks are still sterile.
In my own opinion, why going for expired blank antibiotic disc. Go for unexpired sterile plain disc and embed your plant extracts. You can equally perforate filter paper, sterilize and use.
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