To understand the lowest possible detectability of TNF alpha in healthy human, what is the most sensitive technique for TNF alpha analysis?
The reference interval for TNF alpha in normal healthy individuals is 0-22pg/mL. It contains 212 amino acids, so the moleuclar weight will be somewhere in the neighborhood of 20,000 dalton. It would be a difficult challenge to develop a reliable and robust LC-MS/MS that can analyze TNF alpha at such low concentration. You will probably get a lower limit of detection using some type of immunoassay than using LC-MS/MS.
The lab where I work uses a quantitative multiplex bead assay on Luminex platform for TNF alpha analysis.The test requires a critical frozen specimen.
The reference interval for TNF alpha in normal healthy individuals is 0-22pg/mL. It contains 212 amino acids, so the moleuclar weight will be somewhere in the neighborhood of 20,000 dalton. It would be a difficult challenge to develop a reliable and robust LC-MS/MS that can analyze TNF alpha at such low concentration. You will probably get a lower limit of detection using some type of immunoassay than using LC-MS/MS. The lab where I work uses a quantitative multiplex bead assay on Luminex platform for TNF alpha analysis.The test requires a critical frozen specimen.
The reference interval for TNF alpha in normal healthy individuals is 0-22pg/mL. It contains 212 amino acids, so the moleuclar weight will be somewhere in the neighborhood of 20,000 dalton. It would be a difficult challenge to develop a reliable and robust LC-MS/MS that can analyze TNF alpha at such low concentration. You will probably get a lower limit of detection using some type of immunoassay than using LC-MS/MS. The lab where I work uses a quantitative multiplex bead assay on Luminex platform for TNF alpha analysis.The test requires a critical frozen specimen.
Is it possible do detect 5pg/mL concnetration using ELISA or is better to use alpha ELISA technique
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