There are a lot of differences between protocols! Do you have any experience with or insights into the following:

Food and/or water deprivation: Why do some protocols only say water deprivation over night and not food also? You would think that food stuff in the stomach would slow the uptake. 

Fasting time: How much time is really necessary? There are relatively large welfare and stress issues with fasting, and some protocols say up to 9 hours (overnight). Others say 4-6 hours. And there is a difference between starving during the night, where they do most eating, and starving during early morning/day, where they do not eat anyway.  so is it ok to starve them in the morning (food)?

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