There are a lot of differences between protocols! Do you have any experience with or insights into the following:
Food and/or water deprivation: Why do some protocols only say water deprivation over night and not food also? You would think that food stuff in the stomach would slow the uptake.
Fasting time: How much time is really necessary? There are relatively large welfare and stress issues with fasting, and some protocols say up to 9 hours (overnight). Others say 4-6 hours. And there is a difference between starving during the night, where they do most eating, and starving during early morning/day, where they do not eat anyway. so is it ok to starve them in the morning (food)?