i need to differentiate between neuron and microglia in human brain
The microglia are just resident macrophages. the same nuclear marker(s) for peripheral macrophages will apply to the microglia. thanks.
Hi
you can use Immunohistochemistry (IHC) protocol with Iba 1 antibody for differentiation microglial cells among other neuronal cells
Regards
Sall1 has been shown to
be a microglia-specific transcription factor:
Article Sall1 is a transcriptional regulator defining microglia iden...
http://www.abcam.com/neuroscience/microglia-markers
TMEM119 TMEM119 is a cell-surface protein and a specific microglial marker for both mouse and human. Unlike other microglial markers, this marker has the advantage that is isn’t expressed by macrophages or other immune or neural cell types.
Mouse brain tissue sections stained with anti-TMEM119 (green) (ab209064).
Iba1 is the best marker for microglia. Please see Islam et al., 2017, Neuroscience 340:201-217
Anti-CD11b/c antibody [OX42] (ab1211)
- Mouse monoclonal [OX42] to CD11b/c
- SpecificityRecognizes a common epitope shared by CD11b (ITGAM) and CD11c (ITGAX). It reacts with all macrophages in the peritoneal cavity, activated macrophages and Kupffer cells, but with only ~35% of alveolar macrophages. It also binds to all granulocytes and to dendritic cells, brain microglia, sparse thymic cortical cells and epidermal Langerhans cells. The antibody inhibits activity of the complement receptor type 3 (CR3) (PMID: 3512425).
Neurons are easily identified by general staning methods but if U identify glial cells than U use (IHC) Immunohistochemistry techniques
And use different marker for Microglia use Iba,- 1 and neutral marker NeuN & MAP2 etc
Neuron nuclear antibody as mentioned is wonderful in human tissue for neurons (can reveal both nuclei and dendrites in some populations). I like IBA1 for microglia but that staining is not nuclear.
PU.1
Most of the other markers suggested here are actually not nuclear.
In our lab, we routinely sort fixed nuclei isolated from brain tissue and use NeuN and PU.1 to differentiate between Neurons and Microglia. Specifically we use DAPI+ for nuclei, NeuN AF-488 and PU.1 AF-647. Works like a charm.
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