Ipsita Chatterjee

5 Questions 2 Answers 0 Followers

Questions related from Ipsita Chatterjee

How do I isolate intestinal epithelial cell from mice colon ?

In my experiment I would like to isolate epithelial cells from intestine to do RNA seq. I tried by vigorous shaking of the colon at 37o C, in buffer (DMEM, EDTA, DTT, FBS) for 10 minutes, followed...

09 September 2018 1,629 0 View

Hi, Could anyone help me to analyze my images with AB-PAS stain of small and large intestine?

how to calculate no of goblet cells , thickness of mucus layer and if th r r any other parameters to look at

04 April 2018 4,883 3 View

Gut permeability assay protocol?

There are a lot of differences between protocols! Do you have any experience with or insights into the following: Food and/or water deprivation: Why do some protocols only say water deprivation...

03 March 2018 2,134 1 View

Can anyone suggest nuclear marker for micriglia?

i need to differentiate between neuron and microglia in human brain

11 November 2017 6,119 13 View

My positive control is working but samples(should be gfp positive) are not giving GFP postive band.internal positive control working for all,why?

GFP band size1kb IPC band size 324 kb PCR is already standardize...positive control is giving band for both IPC and the transgene(GFP)

04 April 2016 9,653 2 View