The 96 well plate holds up to 300 ul/ well, do not use less than 100 ul total volume cause cappilary forces suck the liquid from centre of the well. IMHO reader with shaking and kinetic reading might be very beneficial. Anyway have fun and let me know how it went.

The Thermo Scientific Pro-Detect Rapid GST Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of glutathione S transferase (GST)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the GST tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect GST-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling

Features of the Pro-Detect Rapid assay kits: • Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target • Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes • Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations • Specific—assay does not cross-react with common lysis and protein preparation reagents • Reliable—results are similar to other antibody immunoassays, including western blot and ELISA • Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

You can use the final concentrations of an "usual" GST cuvette assay; just adjust the volumes.

One important point that many people neglect is: the pathlength. Usually the calculation of GST activity in the assay with CDNB uses the molar extinction coefficient of 9.6 mM-1 cm-1 for the GSH-CDNB conjugate. Note that the unit (mM-1 cm-1) assumes that absorbance readings were made through a 1 cm pathlength. However, in microtiter plates the pathlength depends on the volume inside each well. Thus, remember to correct for this. Some equipments have a built-in "Pathcheck" features that automatically corrects this.

Good luck,

Daniel