I'd like your advice on Pyrocystis Fusiformis growth. I purchased a culture a month ago and began cultivating it right away. Things, on the other hand, do not appear to be moving forward. There has been no bioluminescence observed yet, and the algae's growth has been poor. The culture was maintained at a pH of 8.2 and a temperature of 20 degrees Celsius with a 12-12 dark/light cycle. Cell counting under a microscope was used to monitor the algae's progress, but because I don't have much experience in the field of microbiology I wasn't sure of my count. I've attached a photo of what I see under the microscope, and I'm not sure if it's algae cells or not.
I'm hoping you could give me some suggestions on how to improve the growth of algae and its bioluminescence.
What nutrient media are you using? Is the light to strong or weak? How big is the culture and how big was the inoculum? These are all things that will influence the growth of the organism.
Barry C Smith F/2 media was utilized, along with a quite bright LED light (5000 lux measured using a lux meter app on the inoculum surface). The culture volume is 4L, while the inoculum volume is around 6L.
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