Hello,
I'm planning on doing a biocontrol study on Erysiphe alphotoides/ syn. Microsphaera alphitoides/ Powdery oak mildew, but first I need to isolate the fungus. Perhaps someone knows what sort of medium is best suited for this fungus?
Dear, Doroteja Vaitiekunaite,
The fungus Erysiphe alphitoides is an obligate fungus. So it can not be isolated on the artificial media in the laboratory. Therefore, you must complete your experiments on the living plant host (oak).
All powdery mildew diseases are caused by obligate fungi like Erysiphe, Uncinula, Leviellula, Phyllactenia, Spodoshaera, Microsphaera etc. All these pathogens are obligate parasites and they can not grow and reproduce far from the host plants.
Yehya A. Salih Thank you for the answer. If I was to add oak leaves to the culture medium, do you think the fungus would still be unculturable?
Hi dear, Doroteja Vaitiekunaite,
Even you add oak leaves to the medium, this obligate fungus can not grow, because this fungus can not grow easily far from the living host plants. The obligate parasite fungi like the causal agents of powdery mildew can not grow on the different artificial media.
The fungi divided into five kinds:
1 - Obligate parasite: The fungus can not grow on the artificial media, but it can
grow and reproduce on living hosts only.
2 - Facultative parasite: The fungus can grow on the media
3 - Facultative saprophyte: The fungus can grow on the media
4 - Obligate saprophyte: The fungus can grow on the media
5 - Symbiotic fungi: The fungus grow with other hosts without harmful such as
mycorrhizae and lichens.
Check https://hal.archives-ouvertes.fr/hal-01102701/document
Also check https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155344
Also check on this paper
https://www.mdpi.com/2073-4441/10/6/695/htm
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