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I am going to perform IC 50 in MDA-MB-231 cell lines in 96 well plate by MTT assay . But before that, I want to confirm the density of cell that i should use to plate. The assay for that is to perform a growth curve by plating different densities of untreated cells and measuring the absorbance  cell. However, i am not sure of the exact protocol. Can someone tell me the protocol with MTT? I know the mtt protocol but i need to know the steps for performing the growth curve with the cells. My assay will be 4 days longs from seeding day (day 0) to treatment day (day 1) and reading day (day 3). Thanks 

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