I have problems growing the bacillus subtilis strain I got from ATCC. I used nutrient broth/agar as recommended by ATCC. I tried both 30C and 37C growth, it was alright on agar, but grew really slowly in broth, and the OD didn't go above 1 after 24 hours. Anyone has experience growing this particular strain?
Hi sir you can try LB broth for the production of bacillus species. If possible increase temperature to 40 c .
The strain should grow fine on most of the common bacterial medias at 37C. There may be an issue with this sample. You could ask ATCC for another sample or request it from a different collection. http://www.straininfo.net/strains/5143
I agree with Chris Dunlap; this is the type strain of B. subtilis and should grow with a doubling time of about half an hour in nutrient broth, TSB, LB, BHI, and so on, if incubated at 37°C with aeration. The Bacillus Genetic Stock Center accession 3A1T is an equivalent strain.
I admit that I'm puzzled that Bacillus subtilis isn't growing for you. I get an OD600 ~ 4.0 with an overnight culture of this strain in LB at 37°C with shaking. You could try adding MnCl2 to a final concentration of 10-5. If your medium is deficient in Mn++, it will not be able to utilize all of the available carbon sources before it reaches stationary phase.
Don't waste time trying to figure it out. Tell ATCC it is not growing and get another sample or contact another collection to get the same isolate. I've gotten hundreds of culture collection samples and it is not uncommon that 10% or so won't grow, are contaminated or are the wrong strain. The irony is I commonly find Bacillus as a contaminant in culture collection samples, because it forms very stable endospores and can contaminate your lab if your not careful.
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