29 January 2025 0 5K Report

What acridine orange reference do you recommend for autophagy in cancer cell visualized under a microscope and flow cytometry?

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How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

In silico techniques for identification of inhibitor/activator/substrate activity?

Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...

08 July 2024 4,724 4 View

Any new data on DMT1/2 Covid-19 Omicron Survival?

Still alive but new info from last hospitalization a week ago. Per Endo ... Covid remapped the electrolyte panel. A high potassium 5.5 actually is low and the patient needs K+ Blood glucose at...

21 May 2024 4,780 0 View

Does anyone have experience with studying GSK2881078 on muscle cells?

The past few months I have started testing the selective androgen receptor modulator (SARM) GSK2881078 to study its ability to increase muscle growth and/or counteract muscle atrophy. I have done...

20 May 2024 8,747 0 View

Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide?

Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide? What is the protocol?

16 April 2024 1,409 1 View

Is it necessary to use a normalization microRNA in each qPCR plate?

For validation by qPCR of several microRNAs, is it necessary to measure normalization miRNAs in each plate in addition to the miRNAs under study? Or can you make plates of microRNAs and then...

15 April 2024 6,393 1 View

How can I make a baseline correction of a FT-IR?

Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...

25 March 2024 7,648 0 View

Cómo se hace los "Traits en PopArt?

I need a little help, how do you make "Traits" in PopArt? I need to make a network of haplotypes on a map

14 March 2024 2,316 0 View

Protein modeling programs with a free commercial license?

Hello, I wanted to ask for help with protein modeling programs (not servers) with a free commercial license. thank you

13 March 2024 6,173 0 View

How to make a 33bp deletion from a plasmid?

I would to delete up to 33 bp from a plasmid to generate deletion mutation. Has anyone tried using Agilent Quikchange lightning site direct mutagenesis to delete such a long region? How feasible...

06 March 2024 8,136 5 View

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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

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Why do we equate male and female arousal?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

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How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

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