Greeting to all academia colleagues

More Chou-Yi Hsu's questions See All

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Why the lateral resolution of OLS5100 (Olympus) can be down to 120 nm?

The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...

15 May 2024 1,458 3 View

Why does the EPR spectrum of MnO2 powder exhibit such wide peaks?

As known, the spin exchange coupled Mn4+ leads to a narrowd singal, at ca. g=2.006 with the line width about 500 gauss. Why the birnessite MnO2 dispalys such wide spectrum? Is any other exchange...

23 April 2024 6,351 3 View

What is the standard electrode potential of the silver wire reference electrode?

The silver wire has been used as a reference in an organic electrolyte in many artcles. What is the standard electrode potential of the silver wire reference electrode and how to calibrate its...

14 April 2024 8,052 3 View

Iso 29134 pdf?

I'm just looking for iso 29134 pdf, does anybody have any idea where I can download it for free?

08 April 2024 6,507 0 View

What techniques can enhance the inference performance of DNN and be used in edge computing?

Edge computing is a decentralized computing infrastructure that brings computing power closer to the data source, reducing latency and improving efficiency. Traditionally, computing tasks were...

19 March 2024 1,229 0 View

IP beads for mass spec. Can i freeze the beads?

I need to submit some IP protein G beads for mass spec analysis. However, the mass spec has decided to be not available. Can I freeze the IP beads at -20C ready for the mass spec? If not, what is...

17 March 2024 7,622 0 View

Why the published article is not in the author confirmation list?

I recently used ResearchGate's Conform Authorship for the first time. When I was checking the box, I found that the article published by Ian Westbry and I was not in the list. I would like to ask...

14 February 2024 7,769 3 View

How to sterilize materials coated on PP in situations where high temperature and UV cannot be used?

How to sterilize a polymer grafted with a photoinitiator before cell culture?

23 January 2024 9,548 2 View

What potential by-products may occur in the EDC/NHS reaction?

I want to use carboxymethyl cellulose for an EDC/NHS reaction to attach amine-functionalized small molecules. Initially, I used an EDC:NHS ratio of 1:1, but the grafting efficiency was low. Later,...

11 January 2024 6,873 1 View

Would you mine to tell me the style of citation to add to my citing tool ?

in the text it is cited in square bracket. in the reference list, it is in the order; author/s, title, journal, year, issue(volume), page. or DOI eg. Smith JJ. The world of...

09 August 2024 9,355 1 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

How can I find my published papers' total read, citation or any other scores?

21 July 2024 9,802 3 View

Need help is coverslipping-Histology samples?

Hello, Our lab has been experiencing sporadic issues with coverslipping. We use the Sakira Tissue Tek Prisma H&E Stainer and a Tissue Tek Glas2 Automated Glass Cover slipper. Periodically,...

12 July 2024 7,016 3 View

Unethical or Illegal? Reviewers Requesting Self-Citations in Unrelated Manuscripts?

Dear Friends and Colleagues, I have a question regarding the review process for journal submissions. When we submit an article to a journal, is it illegal for a reviewer to provide authors with a...

09 July 2024 7,557 7 View

Why citations are not recorded on my profile researchgate web?

Dear colleges , I would appreciate if you have a time to help. I received mails told me there are numbers for research articles. These citations are not recorded on my profile. Regards, Sherif

09 July 2024 6,195 1 View

How does proof comprehension among students change over their academic years, and what factors influence this progression?

Please provide necessary citations. Thank you.

08 July 2024 3,305 0 View