What is the best approach to test graphene oxide antibacterial activities? Is there possibilities that graphene oxide can stick to the plastics (the falcon tube) that we use, that possibly reduce it antibacterial activities?
May be you can use the plating methods which means pour the melting TSA with the bacteria suspensionmixed . And then make GO drop on the center of the TSA before it become solid. After days, you will find the inhibition zone on the plat if the good antibacterial activities.
Graphene oxide is not as hydrophobic as carbon nanotubes so they may not stick to containers the same way.
You need to decide what the best way is based on the question you want your study to answer. Are you thinking of effects on planktonic cells or cells in biofilms attached to a solid substrate? I recommend reading the paper attached here to see other things you should consider (e.g. GO concentration, dispersion media and state, etc.)