Graph of substrate concentration vs. initial velocity does not conform Michaelis Menten graph

01 January 1970 1 8K Report

I was performed a kinetic assay of a fusion phosphatase with DiFMUP. I did this in triplicate and obtained a graph of substrate concentration versus initial velocity. However, the graph doesn't look like the Michaelis Menten one. What could be the possible reason for this difference?

Ross La Haye

It doesn't seem that far off to me. Without knowing anything else I would suspect this is just due to random variation in your data.

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