Good protocol for ICC/IF for cultured cells

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Best pH Strip?

Which pH Strip is the best for checking mammalian cell culture media? Any suggestions?

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How to ascertain/check the quality of MilliQ or ELGA water?

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02 March 2016 3,643 3 View

Compensation in flow cytometry

Can I compensate with beads for my experiments. I use 4 colours in a single tube (FITC, PE, PerCP Cy5.5, APC). What does it matter if I use beads for my compensation. Why can't I just use beads to...

02 March 2013 5,340 4 View

Trypsin edta

Would anybody know if there is any difference in using DI-Sodium and TETRA-Sodium EDTA in the preparation of 0.25% Trypsin - 0.53mM EDTA? Thanks!

31 December 2011 2,629 0 View

Can I change concentration and brand of antibodies mid-experiment?

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Sodium azide in immunohistochemistry?

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Is perfusion required to stain brain tissue with every method?

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Help with mounting meningeal tissues with Prolong Diamond?

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Air bubbles forming underneath tissue during dehydration step of IHC?

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Shandon Sequenza leaves my IHC samples with spots that have not been stained?

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Wilfredo Alejandro González-Arriagada

Maybe the Dr. Débora Campanella Bastos (http://www.researchgate.net/profile/Debora_Bastos/) can help you. Try to write to her.

Shahram Pour Beiranvand

Dear Sam

You can find some protocol in http://www.abcam.com/index.html?pageconfig=popular_protocols or http://www.ihcworld.com/ and....

Gayathri Devaraja

Have you developed any specificity checking tools? And the above website would help you.

Sam vijay kumar .J

Thanks folks all of you!

Snow Nie

A good protocol: https://www.creative-bioarray.com/protocol/immunohistochemistry-protocol.htm