Best cell lysis solution for DNA extraction by manual method?

Could anyone help me find the best composition for cell lysis solution for manual DNA extraction using blood as the source? I want to prepare the solution in house. Does anyone have experience...

10 November 2018 2,913 5 View

Best pH Strip?

Which pH Strip is the best for checking mammalian cell culture media? Any suggestions?

05 June 2018 6,271 0 View

How to ascertain/check the quality of MilliQ or ELGA water?

We have an in-house ELGA water system (something like MilliQ water system). We need to check its quality? Can we do it in-house? If so, how?

02 March 2016 3,582 3 View

Compensation in flow cytometry

Can I compensate with beads for my experiments. I use 4 colours in a single tube (FITC, PE, PerCP Cy5.5, APC). What does it matter if I use beads for my compensation. Why can't I just use beads to...

02 March 2013 5,259 4 View

Trypsin edta

Would anybody know if there is any difference in using DI-Sodium and TETRA-Sodium EDTA in the preparation of 0.25% Trypsin - 0.53mM EDTA? Thanks!

31 December 2011 2,575 0 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View