How to modify Al2O3 surface to make it hydrophobic?

Dear Scientists, I am going to disperse Al2O3 with synthetic oil (hydrophobic). Therefore how to modify Al2O3's surface to it stable in the lubricant? thank you very much. Best regards, Agus.

25 August 2020 4,485 3 View

How to determine atom% from weight% as shown in Maud Crystallographic Software ?

My understanding is atom% can be obtained from weight% by dividing all latter values by the appropriate atomic weights and normalizing their sum to unity. However, I am having a difficulty in...

17 June 2020 5,309 5 View

How to preserve shrimp hemolymph?

Can anyone suggest how to preserve shrimp hemolymph sample during transport to laboratory from a remote shrimp farm? How to prevent hemolymph to clot between individual extractions in a pooled...

01 February 2018 5,287 1 View

Why DNA plasmid have to dry after wash by ethanol??

09 September 2017 7,373 4 View

Nitrification vs mineral concentration

Is nitrification directly affected by major minerals (Ca, Mg, K, Na) concentration in water? Does the nitrifying bacteria need these minerals significantly?

09 August 2017 2,095 3 View

Why should I use a reversed column for better peak in HPLC?

27 March 2015 8,813 3 View

How can I determine the stability of biosurfactant based on the emulsion activity test?

I have done a test of emulsion activity (and surface tension) to characterize the biosurfactant produced by bacteria. The emulsion activity reduced after 24 hours, but no more than 10%. How can I...

08 August 2014 9,155 9 View

Co-Immunoprecipitation problem - Can anyone lend their expertise?

I want to purify a protein complex with co-IP kit (Thermo-Pierce, resin column), but, at the end, I could neither elute enough specific protein nor visualise by WB using antibody against...

03 August 2014 6,377 6 View

Determination of Ginkgolide A and B using HPLC

Can anyone suggest a suitable solvent system in HPLC (using column of Licrhospher 100 RP-18 5um, Merck, Darmstadt, Germany) to determine Ginkgolide A and B ?

28 November 2012 4,169 18 View

Can you explain how to develop a good questionnaire?

A questionnaire is an instrument usually used in survey research. How to make a good questionnaire so that it can provide us the best results (valid, reliable) to our study?

12 September 2012 3,699 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View