Does anyone have a good antibody for Fsp1 (S100A4) that can be used for IHC on frozen sections? Thanks so much!
You can use a ready-to-use pathnsitu antibody; it will yield good results.
What quantitative techniques and measures can be used to evaluate the relationships between parents and their adult children?
23 January 2024 9,931 1 View
I want to understand all the possible causes, how different Canadian cities try to alleviate houselessness, how addictions are addressed, and how mental health challenges are addressed for our...
19 November 2023 5,829 3 View
I'm working on a government form-building service to help public servants quickly and easily get their forms online. I know many folks in different jurisdictions are working on this same problem....
22 February 2023 8,109 0 View
For difficult to transduce adherent cell lines, I was wondering if anybody has tried spinoculation with polybrene? If so, what is the protocol you used?
27 November 2022 6,147 3 View
Do you know of research that suggests the number of non-fatal suicide attempts to actual fatalities i.e., in a period of time and in the US, another country or worldwide?
13 January 2022 1,739 3 View
The following HHS factsheet -- see link below & attachment - states that one can help prevent suicides by ASKING a person-at-risk directly if they have thoughts or plans to kill themselves;...
02 January 2022 415 3 View
I have two unique teleost species, Saffron Cod and Broad Whitefish. I am analyzing the HSP70 concentrations in the cranial, hepatic, and muscle tissues. The Broad Whitefish samples all worked...
17 November 2021 3,077 2 View
I am looking for a protocol to select Pichia colonies that are expressing an extracellular protein. The paper I'm following transfers the Pichia colonies to a nitrocellulose membrane and then...
02 August 2021 1,192 3 View
I would like to test my Primer for future Real Time PCR and my lab only has an Applied Biosystems ViiA 7 Device (a Real-Time One-Step RT-PCR experiment). Unfortunately, I haven't worked with this...
18 April 2021 2,792 1 View
Does Research Gate have a paper/hard copy equivalent?
01 November 2020 637 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View