Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

Examples of SWAT used in water management in operational practice?

Do you know any examples when the hydrological model made using SWAT became some software product used in operational practice for water management (cases of the water reservoirs management are...

04 June 2020 4,191 1 View

What are these cells with highly condensed basophilic nuclei and a light cytoplasm between proximal tubule epithelial cells in the zebrafish kidneys?

Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...

03 June 2020 4,093 5 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

No title

It is quite difficult to find these enzyme preparations at that moment. Can anyone tell me who I can contact on this issue with?

29 February 2020 2,130 3 View

How to dilute fish blood plasma?

We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...

29 September 2019 8,819 3 View

Does anybody know a database on fluorescent properties of chemical compounds?

Does anybody know a database on fluorescent properties of chemical compounds? There are many papers describing flurescent properties of various compounds. Is there any database on fluorescent...

06 August 2019 9,239 3 View

How shoud one switch off a rotary vane pump?

How shoud I switch off a rotary vane pump? Should I initially turn off the pump and then open the shutter to normalize the pressure? Or the order of the actions should be vice versa?

06 August 2019 9,982 3 View

Can anyone verify whether this is an artifact or a point mutation in these Sanger sequencing results?

Hello! I have really strange results in my sequencing chromatograms. The goal was to analyze a point mutation which is heterozygous (DNMT3A gene, in position R882). So I performed a Sanger...

17 May 2019 7,386 3 View

Is it allowed to publish the same article in two journals in two languages?

I planned to publish my new article in one Russian language journal, but in this case I have much less readers than if publish it in a foreign English language journal. As I understand I should...

18 October 2018 553 12 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View