How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Is there any general way to make polymer hydrophobic?

Now I try to make hydrophobic pillar array using Resin 3D printing (Formlab's 'Surgical Guide Resin'). But actually I don't know exactly what the resin is made of. But as we know it is the mixture...

05 July 2020 3,860 5 View

What is difference between GOQDs powder from freeze drying method and evaporation drying method?

My Graphene Oxide Quantum Dots(GOQD) are dissolved in DI water. So far, i used freeze drying method to get the GOQD powder. But it takes little long time. So i want to used evaporation drying...

10 March 2020 7,644 3 View

What is the best mesh fineness in calculating stress intensity factor with FE analysis?

Computed stresses strongly depend on the mesh fineness at a notch, but it seems to be not so strict for stress intensity factors. What is the best mesh fineness, and how about the accuracy in...

08 December 2019 8,810 1 View

How can I get the spread the CNT equally on the glass?

I want to spread the CNT(dissolved in DIwater) equally on the glass. I am using hotplate(100Celsius) now. I drop the CNT(dissolved in DIwater) equally. But After all the DIwater vaporize, the CNT...

03 December 2019 1,320 3 View

How does sublimation temperature change in Ultra-High Vacuum?

We have a molecule that we are thinking of annealing/depositing onto a substrate in ultra-high vacuum (UHV). From our past experience, we know that the molecule sublimates around 90C at 10mtorr....

16 July 2019 1,784 4 View

Why the atmosphere state should be argon gas when manufacture battery electrode?

I read a paper on the manufacture of battery electrodes that said the atmosphere state should be argon gas when manufacture the electrode. Is there any special reason?

27 June 2019 7,855 3 View

Does amplitude reduction factor of EXAFS have a strong temperature dependence?

We performed an EXAFS measurement for some alloys at 20 K. But we couldn't do it for pure elements at 20 K. Does amplitude reduction factor (SO2) of EXAFS have a strong temperature dependence?...

31 March 2019 7,655 6 View

How can i use HPLC for GABA measurement?

Hi. I have a question. I would like to use HPLC for GABA measurements. I would like to use the column with Zorbax Eclipse Plus C18, but I wonder how. Help!

24 January 2019 3,281 4 View

Tiny specks on the walls of wells after cDNA synthesis that won't come down!?

After synthesising cDNA today I noticed some residue on the seal and on the walls. Most of the residue on the top, on the seal, came down upon centrifugation... However om The walls the tiny...

09 April 2018 9,375 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View