Hello everyone!
Can anyone suggest me an appropriate way of making glucose standards using DNS solution for cellulase activity quantification?.
Dear Sofora Jan,
I have attached one article about cellulase activity which on Page 3, the method for preparation of standard solution was completely explained. I hope it would be useful.
I am a 3rd year Computer Science student currently writing our Bachelor's thesis about finding diverse k-shortest paths in pedestrian networks. We have chosen 3 local areas as our proposed...
15 July 2024 4,289 0 View
Penconazole 1. CGA 132465 2. CGA 190503 3. CGA 127841 Fluopyram 1. AE C656948-benzamide 2. AE C656948-pyridyl-acetic acid 3. AE C656948-carboxylic acid I have searched on sigma aldrich as well...
09 July 2024 3,414 2 View
I am sectioning 50um frozen spinal cord samples and trying to stain them with cresyl violet. At the end of the staining protocol, after the samples were washed with xylene, I dry the sample in...
18 May 2024 8,372 0 View
Can I do flow cytometry analysis for CD3,4,8 positive T lymphocytes from the mouse spleen samples frozen in liquid nitrogen and stored at -80C?
10 May 2024 882 1 View
What are names of authors that have writing about technological imaginaries from a non-western, indigenous or historic perspective? I’m especially interested in origin stories from various...
06 May 2024 355 0 View
Dear colleagues, as a part of our study on cyclophilins (Cyps), I wanted to test the well-known and strong interaction of CypA to cyclosporin A (CsA) on isothermal titration calorimetry (using...
23 April 2024 1,230 0 View
The way this index is counted does correspond with the way researchgate says it is counted. I have 1800 reads this corresponds with at least 1800*0,05 = 90 But my score is only 54,7 Explain???
15 April 2024 7,089 3 View
Dear Colleagues, We plan to isolate and characterise extracellular vesicles released by dying cells during radiotherapy of head and neck squamous cell carcinoma. Given the duration of...
14 April 2024 494 3 View
I keep reading that N2B27 media is used in neural cell culture proliferation and stem cell culture maintenance. Do this media maintain pluripotency or does it direct differentiation. which...
31 March 2024 7,297 2 View
Hello! I am currently working on a study using the SOFC model with unresolved electrolyte. I am getting a very low current. If I lower my applied voltage in the cathode terminal, the calculated...
07 March 2024 6,083 0 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.
03 August 2024 3,170 1 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
Hi all. Is it possible to correlate multiple parameters like fruit weight, color, length and diameter, its ratio with water content in coconut ?. Need some guidance on some non destructive methods...
23 July 2024 9,421 3 View
Phase quantification in steel contain BCC and FCC
20 July 2024 8,150 7 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations. Tried twice but similar inconsistency. Like the highest quantity of a biomaterial is...
11 July 2024 2,257 1 View
I have used Bradford assay for protein quantification; however, I am getting inconsistent concentrations for the second time. Like the highest quantity of a biomaterial is having lower...
11 July 2024 3,557 3 View