Does anyone have any experience with glass bottom microplates (96-well)?
They will be highly beneficial for me for the cell based assay that I am working on but they are extremely expensive and I was wondering if they can be cleaned and re-used?
Dear Rose,
Of course, you can clean and reuse the glass bottom microplates. Look at the following steps:
1. Adhered cells can be removed by trypsinization.
2. Remove adsorbed media proteins by treating with 5% SDS solution.
3. Rinse the wells properly with PBS (2X) and with MiliQ water (2x) and dry.
Enjoy reusing it !!!
I hope it suffice your need.
Thank you Abshar!
I knew it had to be possible but couldn't find any protocols for reusing coated glass. As I intend to coat with poly d lysine. Thank you for your protocol!
I am not sure they can go through a sterilization cycle, since they use glue to stick the glass bottom to the plastic well part. Let me know if you manage!
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