Rose O'Reilly

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Questions related from Rose O'Reilly

How to fix PC12 cells uniformly?

I've been struggling for a long time now trying to find the perfect combination of surface coating (collagen or poly lysine or laminin or combinations of the 3), trying multiple fixation times,...

30 August 2017 3,546 6 View

Glass bottom 96-well plates

Does anyone have any experience with glass bottom microplates (96-well)?They will be highly beneficial for me for the cell based assay that I am working on but they are extremely expensive and I...

16 June 2017 939 3 View

I have dancing black dots in my cell culture?

I have experienced dancing black dots in my cell culture, upon finding the little buggers down a microscope I binned them and ordered new cells. I binned all products used with the cell line,...

10 October 2016 6,068 18 View

I have been growing PC12 cells happily in suspension but struggling to get a monolayer of cells when plating in an assay plate. Any suggestions?

I have been trying to produce an assay for the outgrowth of PC12's with NGF. I'm struggling to get a monolayer of cells in a 96 well assay plate coated with poly D lysine and I've also tried a...

07 September 2016 9,593 5 View