How Ribozyme activity could be accurately measured in tube?

Seyed Younes Hosseini @Seyed-Younes-Hosseini

28 July 2023 0 7K Report

I did ribozyme activity measuring by putting IVT transcribed RNA into Mgcl2 and Tris-HCL buffer but not intended band appeared after fragment analyzing. Is there any other condition for better reaction?

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