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QPCR standard curve troubleshooting advice?

Hi all, I am trying to set up my standard curve for qPCR analysis, presence absence of D. septosporum, using a QuantStudio 5 real-time PCR system and associated software. I am using a 5X...

25 November 2020 710 3 View

Can anyone help with setting up the R germinationmetrics Package using multiple species and treatments?

I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to...

18 November 2020 7,160 4 View

How to extract patient specific 3D geometry from GE Vivid E95 3D/4D echo?

I am needing to obtain patient specific geometries of the left ventricle for use in CFD and 3D printing. Does anyone know how to do this using 3D/4D echo obtained from the GE Vivid E95? Does this...

29 September 2020 2,614 3 View

Ideas for knocking down protein that is heavily upregulated in cancer?

Hello all, I am trying to knock down a protein that has been documented to be heavily upregulated in oral squamous cell carcinoma (confirmed via western blot). I have attempted to knock down this...

31 August 2020 9,214 3 View

How to calculate error in photocatalytic data?

Hello, I made some measurements using a UV-vis spectrometer at intervals of 5 minutes to see the degradation of a resazurin dye by TiO2 under UV light. I did not repeat these measurements. How can...

23 May 2020 1,373 3 View

What is the ideal retention interval for measuring retention of knowledge?

If you're using an intervention in classrooms and testing knowledge at pre-intervention, post-intervention (immediately after) and then again at a retention time period, how long is the ideal...

19 May 2020 307 8 View

What is the best way to quantify the amount of a bispecific t cell engager (BiTE) present?

I'm currently working on a project to study BiTEs, but I'm struggling with the best way to quantify the amount of BiTE being produced by my cells. Any help would be greatly appreciated. I've...

23 February 2020 9,189 1 View

What statistic do I use on non-parametric data where I have a response variable and two interacting categorical explanatory variables?

I was wondering whether anyone had any suggestions as to what statistic to use (I use R Studio). I have some muscle cell circumference measurements (response) and I have two explanatory variable...

27 January 2020 5,835 3 View

Can someone please explain the credibility behind Travelodge's survey: "The Secret to a Good Night's Slumber is to Sleep in a Blue Bedroom" ?

Currently, I'm investigating the effect bedroom color has on one's quality of sleep. Many (non scholarly) works I've come across have mentioned / cited this survey, conducted by Travelodge in...

21 September 2019 4,088 2 View

Is there a way to simultaneously visualize DNA and protein following electrophoresis?

I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which...

15 May 2019 9,228 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

How to size battery banks?

I am designing a small grid connected solar PV power-plant. As the battery's capacity is specified in Ah and demand in kWh. What is the best way to size the battery bank? I have battery price in...

28 February 2021 1,638 3 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to sample data to increase presence of solar panels in the images?

I am working on creating deep neural network on geospatial dataset and wanted to know how to do the sampling so that I can increase the presence of solar panels in the images. I cannot use entire...

25 February 2021 3,936 6 View