What is the most controllable (marine) bacterial culture you can cultivate in simulated seawater?

02 December 2020 6,796 3 View

Are there studies of the Effect of Social Media Memes on COVID 19 Information?

Dear Colleagues, I put up this question at RG in order to find out what is being studied about the effects on people of social media memes as they attempt to find reliable information regarding...

17 May 2020 1,398 18 View

Is there a program to pull only nouns or verbs from a text?

Dear Colleagues, I am compiling a list of words for an index and I wondered is this exists--sure it does somewhere. The index is for a collection of literary analysis essays. Thanks, Gloria

25 February 2020 1,755 4 View

Does 36B4 really is a unique copy gene?

02 September 2019 3,570 2 View

How long can the fresh Lentivirus store at 4 degree?

I make lentivirus weekly, but just use 30% of total volume. the leftover is aliquoted as 1 ml each tube then freeze at -80 freezer. Questions: 1. is this store method correct? directly put in -80...

08 April 2019 4,628 4 View

Is there a 6-well plate for cell culture available with a more secure/locking lid?

We are looking for culture plates with lids that do not lift as easily outside the incubator. Alternatively, any techniques people have for how to avoid accidental lifting of plate lids would be...

01 August 2018 9,108 3 View

Is it possible for peroxide values of Ximenia oil to be affected by post harvest handling or length of storage ?

We measured peroxide values of Ximenia americana and X.cafra and the values were very low (3.7). i was just wondering if maybe post-harvest activities might affect this value.

14 May 2018 2,662 1 View

Can anyone help with theory on cultural transmission via science fiction, fantasy, and horror (gothic-based) literature?

Anthropologists generally study behaviors that persist in human groups. The invention of print media gave a new area for the preservation and transmission of cultural values. Literature,...

16 February 2018 6,397 7 View

Anyone working with Gardnerella vaginalis??

Good morning. Currently I am, working with G. vaginalis, but I have some problems to find an optimal medium to grow this bacteria. I used Casman Broth but the optical density of the culture was...

07 February 2018 4,211 2 View

Psychometric methods of testing the factor structure of a scale?

I want to examine the factor structure of two scales respectively (scale A: 14 items; scale B: 26 items). We are trying several methods: CFA followed by EFA; Rasch analysis and IRT respectively....

24 August 2017 2,254 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View