Which variables can be used to increase/decrease the grain size of czochralski-grown polycrystalline silicon?

Which variables (such as pull speed, melt temperature, annealing temperature/duration, cooling rate, etc.) affect the grain size of czochralski-grown polycrystalline silicon? In what direction...

10 January 2021 2,204 6 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Do you have any application suggestion for automated data collection for surveys?

I'm planning to do survey for about more than 700 people online. Is there has any software you can suggest to collect the data? Thanks

05 October 2020 8,079 7 View

Where can I obtain Streptomyces lividans strains for enzyme production?

I am having trouble figuring out where to obtain Streptomyces lividans strains that are commonly used for enzyme production. Specifically I am searching for the following strains: S. lividans...

25 May 2020 6,205 3 View

What devices are available for percutaneous pH monitoring?

I'm interested in percutaneously measuring local changes in tissue pH in vivo with and without a therapy. What devices are available for percutaneous pH monitoring in large animals?

20 March 2020 2,731 3 View

How to genotype between wild-type and heterozygous mutant zebrafish with only 1 bp difference?

22 January 2020 7,703 4 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

Whole Blood Over Recovering Compared to Blood Plasma?

My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...

10 July 2019 7,391 3 View

How can I perform a meta-analysis of incidence rates (X events per 100,000 person years) with 95% CI without a comparison group?

I'm performing a meta-analysis, attempting to produce a summary statistic for incidence rate of events of sudden death per 100,000 person years with 95% CI, and eventual subgroup analysis. I am...

11 June 2019 5,867 5 View

Is there a limit to the amount of Polyvinyl Butyral (PVB) that can be dissolved into ethanol?

Is there a maximum solubility level for ethanol?

11 February 2019 2,793 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View