In order to express a protein by adenoviral transduction in mamalian cells, should I clone genomic DNA or cDNA? Thank you!
cDNA derived from mRNA for your gene of interest is best. The genomic DNA of most genes contains introns which would make amplification for cloning difficult.
cDNA
If there is no intron then both cDNA and gDNA are OK, otherwise you have to use cDNA for gene expression
Thank you for your answers!
Could anyone please let me know also the loading buffer used with Crystal Violet staining.
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