and it showed DNA, though less(130ng/µl) but there was no band on gel. I have run 2 gels; one 0.8%, run at 50V
Daniel M Cohen
What was the OD ratio (260/280) of your gDNA prep? Is it possible that you were mostly detecting RNA? Alternatively, have you considered that perhaps the gDNA is far too large to enter your agarose gel? Perhaps you need to fragment the genomic material in order to resolve it on a gel. You could try brief sonication or performing restriction digestion with a rare cutter with a small amount of you gDNA prep and see if this facilitates detection on the agarose gel.
1 votes
1 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Biological Techniques
More Arpita Ghorai's questions See All
Similar questions and discussions