Hi everyone,
I am currently thinking of doing a genome wide CRISPR screen for potential biomarkers that can predict drug resistance in patients. We have done this with other cancers using adherent cell lines and have had some degree of success so far with finding valuable targets. Recently, we decided to initiate a screen for AML due to a collaboration with a hospital that can provide us valuable patient AML samples. However, because we do not have any experience in leukemia, I would like to seek your advice. Firstly, I have looked for commercially available cell lines and found the following:
KASUMI-1, KY821, KY821A3, Kasumi-6, SKNO-1, NKM-1, KG-1, HYT, NOMO-1, SKM-1, OIH-1
We plan to infect these cell lines with a commercially available CRISPR Cas9 library containing 122000 guide RNAs. However, my question is whether it possible to infect suspension cell lines with our library construct viruses?
Hi Osama,
I will be using the CRISPR-Cas9 system to perform a genome wide knockout.
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