Gene editing knock-in experiments, if off-target occurs, can donor still be knocked in?

14 November 2024 0 1K Report

Off-targeting occurs when the wrong position is cut, so can the donor still be knocked into the wrong position when the homology arm and the sequence at the wrong position are not complementary?

Badges
Science method

Similar topics
Biological Science
Ecology

More Zhou Qiqi's questions See All

Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Stable transfection be combined with two or more antibiotics to screen cells?

Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?

10 June 2024 9,739 0 View

What role can community engagement play in heritage preservation and interpretation?

To examine the impact of community engagement in heritage preservation and interpretation

01 June 2024 3,478 11 View

Can sulfate dextran be a substrate for dextranase?

Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...

23 May 2024 1,471 0 View

[Fairclough’s three dimension (Description+interpretaion) + content analysis ]+ [Fairclough’s (explination) +Willig’s practice +Foucaldian CDA]?

In order to understand whether the two newspapers marginalised one group and diverted attention from other group following a case study. Firstly using first and second levels of Fairclough’s...

23 May 2024 3,208 5 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

21 May 2024 9,684 1 View

Do cultured embryonic stem cells or iPSCs migrate spontaneously?

Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?

18 May 2024 6,861 2 View

Why the lateral resolution of OLS5100 (Olympus) can be down to 120 nm?

The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...

15 May 2024 1,458 3 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Preparation of PC solution?

Hello, everyone, recently I have been transferring two-dimensional materials, using PC transfer, but there is a problem with the configuration of PC solution recently, and I cannot pick up the...

11 May 2024 6,564 0 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Why is this tissue slice different?

This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...

18 July 2024 5,602 0 View

Can we progress towards defining obesity based on body fat content?

The burden of obesity is enormous. The greater challenge lies in accurately diagnosing obesity at the right time, reflecting the true increase in visceral fat. BMI may not accurately represent...

14 July 2024 8,771 4 View