From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such, I have decided to use PCR cloning where I design primers either side of the GOI.

In my paper, I need to include research/evidence that shows why two WRPE downstream and two CMV promoters is a bad idea; however, I cannot seem to find anything in the research -- just my supervisor's recommendations.

Thanks heaps!

More Joshua Nicholls's questions See All
Similar questions and discussions