Joshua Nicholls

6 Questions 4 Answers 0 Followers

Questions related from Joshua Nicholls

ImageJ or ZEN Blue: How to add an intensity scale to an image?

I have performed anti-Oct-4 staining with A488 on a colony of hiPSCs. In ZEN, I have applied an intensity channel (rainbow) to the image. As you can see, the highest intensity is located in the...

24 July 2023 6,797 1 View

Graphpad Prism: How to generate SEM and SD when performing an Area Under the Curve Analysis?

I have generated a peristimulus time histogram from some neuronal electrophysiology data and I am trying to compare the AUCs for several data. When performing AUC, GraphPad does not generate SEM...

26 June 2023 287 1 View

GraphPad Prism: How to compare multiple Area Under the Curve analyses from a Multielectrode Array (MEA) Post-Stimulus Time Histogram (PSTH)?

Using data from 11 wells on a 24-well MEA, I have collected repeated-measures data on the number of events (spikes) occurring +/- 1 second of a stimulation event. We ramped up the voltage from...

16 June 2023 6,976 0 View

Why do fermented foods (such as kimchi and sauerkraut) produce CO2 if CO2 is not a produce of lactic acid fermentation?

Biochem major here. Just a bit perplexed as to why my Kimchi and Sauerkraut produce bubbles when this is not a direct by-product of lactic acid fermentation. Are other bacteria involved, or...

17 October 2022 4,220 2 View

Gene Cloning: What are the potential problems with doubling up with redundant WPRE and minimal CMV promoter?

From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such,...

06 November 2020 8,893 1 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

16 September 2020 9,969 3 View