Joshua Nicholls

12 Questions 4 Answers 0 Followers

Questions related from Joshua Nicholls

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

24 June 2024 3,964 1 View

What should the phase and pulse duration be for deep-brain stimulation (DBS) for epilepsy?

In DBS settings, I have seen stimulus widths of up to 500 us in duration; however, the most common pulse duration seems to be between 90-150 us. Clinically, mono- or bi-polar electrodes can be...

27 May 2024 5,783 0 View

Why does extracellular cathodic (-ve) stimulation depolarise neurones?

I am a bit confused by these statements: "For extracellular stimulations, a cathodic pulse can generate a current sink in the space immediately around the stimulation electrode, thereby...

12 May 2024 3,269 2 View

Should a biphasic electrical stimulation pulse on neural networks be positive followed by a negative phase or negative followed by positive phase?

I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...

10 May 2024 7,145 1 View

How can I better improve the passaging process of hiPSCs, resulting in a purer passage?

I am passaging my hiPSCs with EDTA and E8 medium. However, I have noticed the presence of spontaneously differentiated cells in the final passage. What other techniques are available to improve...

22 August 2023 2,942 1 View

ImageJ or ZEN Blue: How to add an intensity scale to an image?

I have performed anti-Oct-4 staining with A488 on a colony of hiPSCs. In ZEN, I have applied an intensity channel (rainbow) to the image. As you can see, the highest intensity is located in the...

24 July 2023 6,808 1 View

Graphpad Prism: How to generate SEM and SD when performing an Area Under the Curve Analysis?

I have generated a peristimulus time histogram from some neuronal electrophysiology data and I am trying to compare the AUCs for several data. When performing AUC, GraphPad does not generate SEM...

26 June 2023 305 1 View

MultiWell Analyzer: What is trace count?

I extracted PSTH data from the MultiWell Analyzer. We recorded a 5-minute experiment (300 seconds) with stimulations occurring every 10 seconds (30 in total). On the excel spreadsheet, Trace...

19 June 2023 7,505 0 View

GraphPad Prism: How to compare multiple Area Under the Curve analyses from a Multielectrode Array (MEA) Post-Stimulus Time Histogram (PSTH)?

Using data from 11 wells on a 24-well MEA, I have collected repeated-measures data on the number of events (spikes) occurring +/- 1 second of a stimulation event. We ramped up the voltage from...

16 June 2023 6,987 0 View

Why do fermented foods (such as kimchi and sauerkraut) produce CO2 if CO2 is not a produce of lactic acid fermentation?

Biochem major here. Just a bit perplexed as to why my Kimchi and Sauerkraut produce bubbles when this is not a direct by-product of lactic acid fermentation. Are other bacteria involved, or...

17 October 2022 4,232 2 View

Gene Cloning: What are the potential problems with doubling up with redundant WPRE and minimal CMV promoter?

From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such,...

06 November 2020 8,920 1 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

16 September 2020 9,992 3 View