Does someone know about Gateway efficiency?
I mean, if I use 150ug DONR plasmid and 150ug Destination plasmid, finally, how many plasmid really take place the LR reaction ? or percentage?
I've tried to find some info from Gateway paper, but nothing could find about efficiency. Only found is "An efficient LR recombination reaction will produce >5000 colonies if the entire LR reaction is transformed and plated."
Maybe you have some ideas about this.
Thank you for any help~