How to insert a simply supported boundary condition without in-plane fixation in ANSYS (Transient sructural)?

Dear all, I have a question regarding imposing a boundary condition in ANSYS (Transient Structural). The problem is a slamming of a composite plate in water (FSI problem). The geometry of the...

27 October 2020 640 3 View

Gelatin nano particles, obtained by two-step desolvation, do not re-disperse in water. What is the problem?

Hello I have synthesized gelatin type A nano-particles by two-step desolvation. After 4 days of freeze-drying, the powder was kept in the freezer (-80 C). Today I tried to redisperse the...

18 February 2020 9,494 10 View

Recombinant protein production in insect cells using plasmid-based system?

Is it possible to produce a Flag-tagged catalytic domain of a murine enzyme in insect cells using a plasmid-based system? does it affect the functionality of the final enzyme?

29 November 2019 7,504 3 View

The small dimension of gelatin nano gel. what is the problem?

Hi I am trying to Synthesis gelatin (type A) nano particles by two-step desolvation method. By DLS, the dimension of gelatin particles are less than 50 nm. I am not sure what is wrong with my...

23 July 2019 964 3 View

What are the best polynomial approximation functions for analyzing free vibration and impact analysis of plates?

I want to do a free vibration analysis for a plate. In this research, I want to use the Ritz method. For Ritz-shape functions, which are the best polynomial functions that give the most accurate...

10 April 2019 2,612 5 View

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, biologists use absorbance even more than 1. Is it correct?

In MTT or Alamar Blu test for cytotoxicity assay by using UV spectrophotometery, I saw many biologist researchers that use absorbance even more than 1 for their calculations. Is it correct? As I...

10 March 2019 4,442 3 View

To make flexible polyurethane foam, necessary to observe the mixing process?

20 June 2016 8,595 7 View

How much GAGs (glycosaminoglycans) is expected of MSCs in their ECM?

Hello, Does anyone know how much GAGs I can collect if I decellularize MSCs in their stemness state and can I stain this GAGs with Alcian Blue? Thanks

23 January 2016 2,565 4 View

What is the integrin binding site for Vitronectin?

As we know vitronectin and some other proteins can bind to different types of integrin. I want to know about the binding site in Integrin which the vitronectin with a RGD-sequence can interact...

30 January 2015 5,378 5 View

How can I extract nodal values of displacement, coordinates and time of each material point at every increment of time in ABAQUS/EXPLICIT?

How can I extract " nodal values of displacement, coordinates and time of each material point at every increment of time" in ABAQUS/EXPLICIT?

30 January 2015 2,597 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View