What could we change in preparing tissue samples prior cryosections(cryostat) method for more convenience and results? ( I mean cryo-embedding) The second question is in which areas of research do you use this one and why is it a pretty rare method of imaging? It is interesting to get answers from researchers who are working currently with this method or those who worked with that recently.
Thank All in advance for any ideas.
Hi Dimitri;
As our lab does immunochemistry and histoenzymatic I can tell you by personal experience that frozen sectioning improves results in many ways; as the tissues are not fixed, epitope retrieval steps are not needed in much cases. And the histoarquitecture is pretty well conserved, endogen eznymatic activity is also conserved.
hope it helped!
Dear Dimitri, I agree to Oscar in the staining results, given that you can live with the worse preservation of morphological details in frozen sections.
I prefer frozen tissues especially for fluorescence microscopy. You have to be on your guard though when harvesting your samples. They are not fixed and if they are nor frozen properly and quickly the tissue will suffer.
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