What could we change in preparing tissue samples prior cryosections(cryostat) method for more convenience and results? ( I mean cryo-embedding) The second question is in which areas of research do you use this one and why is it a pretty rare method of imaging? It is interesting to get answers from researchers who are working currently with this method or those who worked with that recently.
Thank All in advance for any ideas.
Frozen sections are used in deciding the limit of surgical excision of lesion clinically
Same is used to study the dental plaque images without disturbing plaque
Rapid and sufficient cooling is desirable to cut sections
More training is required to assess the unstained sections as everyone is habitual of stained sections visualisation
More improvement is needed to diagnose the images without stains by modifying images digitally
Hi Dimitri;
As our lab does immunochemistry and histoenzymatic I can tell you by personal experience that frozen sectioning improves results in many ways; as the tissues are not fixed, epitope retrieval steps are not needed in much cases. And the histoarquitecture is pretty well conserved, endogen eznymatic activity is also conserved.
hope it helped!
Dear Dimitri, I agree to Oscar in the staining results, given that you can live with the worse preservation of morphological details in frozen sections.
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