I want to use a contact lens for rabbit for transplantation of stem cells to the corneal surface with substrate. i want to put a contact lens which can also able to diffuse gas (oxygen and CO2). I want to use this lens just to avoid the problems due to blinking. Please suggest me from where I can get this. Please also suggest me, what other methods would be possible. As I am also not able to suture the cell-substrate sheet. I also would like to get information about other ways to put the cell-substrate patch over the cornea other than chemically defined bioadhesive. Please do help in this. Thanks!!!
hello !
You can try to put a soft contact lens for human use with smaller diameter.
Depend of on the rabbit kind an age, the corneal curve and eyeball diameter vary, So, a nice try could be made on adult rabbit. Before the action, put the Tetracaine 0.5% eye drop in the rabbit eye (and prepare the artificial tears for later frequent administration). Also, a third eyelid will be a problem during the placing the lens, so you have to ask an assistant to hold the eyelids.
For an adult rabbit, 5 kg or greater, you want a silicone hydrogel lens, such as O2 OPTIX from Alcon. Most likely need 8.4 - 8.6 mm diameter. A thicker lens such as a -3.0 OD lens should stay in the eye better. The nictitating membrane should not be a problem. Years ago we used to excise the nictitating membrane but risk developing an infection. Also, the lens should be fairly flat.
If nictitans is a problem, you can suture across the top of the implanted test material. Vets treating epithelial defects in cats and dogs often perform this.
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