Hello guys,
currently i am doing a project on antibacterial effects of Pandanus amaryfollious (leafy parts) and Cucurma caesia (Legume parts). I have both aqueous and organic extracts of the plant sample. Both samples are now are in liquid state.
[The organic extracts were treated under rotary evaporator to remove the organic solvent (Choloroform:Methanol)]
However, I am trying to remove the moisture or water content using freezer dryer, but this consume a lots of time and seems like not feasible to me.
I am planning to use vacuum filtration where i crystallized some of the extract, then treat it under dissication method where the water will be absorb by the absorbing agent, leaving a dry crystal or powder.
Is this okay?
Thank You.
By means of crystallization, I plan to immerse the flask containing the sample with ice (Ice on the outside of the flask), to form minute crystals which are obtainable.
Hi,
It is not a good idea. the reason is,
I hope your are doing the research not commercial. secondly the compound which you want test for antibacterial either unknown or not phytochemically validated.
As per me, the freeze drying is the best option and right option to proceed further.
Because, if you do the vacuum filtration you can not sure what compound retained and what compound passing the filtration aid. unless you were not sure about molecule you looking for.
In crude extract most of the time impurities crystallize first not active compound( means plant fibers and lignants crystallize first in cure extract)
so try to freeze dry the whole extract and use for your study then try to screen and isolated the most active or desired compound.
all the best
Attached article may help you
Article LYOPHILIZATION / FREEZE DRYING – A REVIEW
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