I am looking for a standard ?

Dear Researchers, I am a researcher and I am working on a Xenon accelerated aging of a polymer and I need the ASTM G 155 standard. I will be grateful if someone can share it with me. Best...

28 December 2020 549 2 View

Explain the strain determined using directly the tensile machine test or the video extensometer ?

Dear Researchers, I would like to know the logical margin between the strain of a polymer in the tensile test determined through the traverse (the value from the machine itself) or using a video...

12 December 2020 3,636 4 View

What is the right method to identify microbes from commercial effective microorganisms?

First, how to isolate DNA from effective microorganisms? is it the more to genomic DNA isolation or metagenomic DNA isolation protocol? Second, is the Bacterial 16s rRNA Amplicon Sequencing with...

23 September 2019 8,440 8 View

How to decide the type of a protein from different families given in Blastx?

I have faced this with more than one gene. in the step of gene annotation of my NGS data I found in many case my enzyme of interest but I found that this enzyme is found in multispecies, it means...

08 December 2017 2,680 1 View

Genetic method to detect a gene in bacterial library?

what ca we do if we are sure that one of 100 of bacteria has desired gene (which we have its DNA sequence) and we want to know which one has it? to explain more: I have sequenced 100 fosmid with...

26 November 2017 5,951 4 View

What is the best choice of Polymerase for large DNA PCR?

04 December 2016 3,847 29 View

Can anyone assist me with Fosmid DNA electrophoresis troubleshooting ?

I have run recombinant Fosmids DNA (around 50kb) on agarose gel electrophoresis, I could visualize only smears and I have remarked that the wells contain DNA as well so may be my fosmids did not...

06 November 2016 4,046 6 View

Data analysis software for high throughput screening of hydrolytic enzymes in metagenomic DNA library?

15 February 2016 2,502 3 View

What are the data analyses methods of high throughput screening for hydrolytic enzymes?

15 February 2016 7,108 2 View

What could be used to substitute CTAB?

I am about to perform metagenomic DNA extraction, and one of the extraction buffer composition is CTAB (Cetyl Trimethyl Ammonium Bromide), unfortunately we don't have CTAB here.

13 January 2016 6,286 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How do I add a udf to Ansys fluent and does it need to be compiled before hand ?

I have a Define_CG_Motion udf written in c where do I add it to my Ansys fluent project and does it need to be compiled before that?

21 February 2021 9,347 3 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

How to perform analysis on Chandrayaan-2 DFSAR data using MATLAB or other languages like python?

Kindly suggest any libraries/Books/platforms/tutorials for the same.

04 February 2021 1,708 2 View

How is the variance for an image exactly computed to test the uniformness of its histogram?

I am trying to compute the variance of a plain and cipher grayscale LENA image... I found in a paper that variance value is equal to 630780 for a plain Lena image while if I calculate I find it...

28 January 2021 8,036 5 View

How to do fishnet analysis on non-geological data in R stat software?

Hi All, I am working on a study that would require to measure the movement activity of experimental mice. For this to do, I am monitoring the mice with thermal camera, and I can get the...

24 January 2021 7,399 1 View

What is the best way of processing very large files in python?

I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...

15 January 2021 6,691 4 View

IET Digital Library Access?

Hello to everyone, I do not have access to the IET Digital Library at the institution where I work. Can those who have access to this library send me the publication whose DOI is given below?...

13 January 2021 9,385 2 View