How to calculate the malondialdehyde (MDA) concentration for analyzing lipid peroxidation in the homogenate of organs (liver, kidney …..) from rats ?
Hello,
If the kit is not available you can use a colorimetric method. This method is reproducible.The rate of lipoperoxidation (MDA level) in the homogenat in our case we evaluate the MDA in gastric mucous membrane. the MDA content was assessed by using the thiobarbituric acid reactive substances (TBARS) test (Ohkawa et al., 1979). 100 μL of the gastric homogenate was added to 750 μL of 8.1% SDS, 750 μL of 20% acetic acid and 750 μL of 0.8% TBA. The mixture was incubated in a water bath (Memmert, WB22, Germany) at 95 °C for 1 h. After cooling, the reactants were supplemented with 500 μL distilled water, 2.5 mL n-butanol-pyridine (15:1), shaken vigorously for 1 min and centrifuged at 4000×g for 10 min. Individual values were extrapolated from a TEP (1,1,3,3- tetraethoxypropane) standard curve (mmol/mL) and the results expressed as nmoles (TBARS) per mg of protein (nmol TBARS/mg prot).
I remain at your disposal for any other information of malondialdehyde (MDA) content.
