Formation of Biuret impurity oberved during the synthesis of an Urea derivative of an API. how to reduce/control the Biuret impurity??
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Langyage problem
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I wish to isolate SP-D proteins and I was not sure why the protocol mentions the use of MnCl2 as an elution buffer to elute the SP-D protein from the maltose agarose beads column. Any help would...
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Dear amazing members, I have a doubt. If I have three adjacent planes with different boundary conditions, in a 3D domain, Dirichlet (fixed temperature) on one plane, Neumann fixed flux on...
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Any one please explain this.
05 November 2022 6,569 0 View
Since proteins give positive test for biuret I was wondering could that be the case with glycoprotein? Also do glycoproteins have any general test like biuret?
27 October 2023 8,894 1 View
Hello to everyone. My work focus on studying PTMs and I have successfully phosphorylate my protein in vitro (Checking the reaction products by SDS-Page, all of my protein should be...
29 March 2023 1,640 3 View
Tried with 7.5%, 10%, 12% and 15% gel percentages.. Protein concentration was checked through biuret and it is about 1.7mg/ml.. Used coomassie blue for staining (1hr) followed by destaining for...
19 March 2023 715 6 View
I've always conducted biuret assay by using a spectrophotometer. Recently, I have tried using a micropate reader for it. It is faster and requires less reagent and BSA. But it seems that the...
10 January 2023 736 6 View
Hello. I used from Thermo Scientific BCA kit and Sigma protein STD 2 mg/ml (0-2000 ug/ml) and prepared a lot of dilutions from my sample (up to 1-64). I had a different final concentration on my...
18 March 2020 652 3 View
My wastewater sample is reddish brown in color and is it possible to conduct biuret test for such kind of sample? If not what kind of test would be suitable?
02 March 2019 1,332 1 View
Hello I want determine degree of hydrolysis by measure the percentage of protein soluble in 10 % trichloroacetic acid /total protein in sample, how I can measure the total protein with biuret...
11 December 2016 7,783 2 View
I want determine degree of hydrolysis by measure the percentage of protein soluble in 10 % trichloroacetic acid /total protein in sample, how I can measure the total protein with biuret method?...
29 November 2016 7,969 2 View
Is it possible that using a biuret method to measure protein of suspension mixture?
10 November 2016 8,503 3 View
Biuret content in urea is maximum permissible at 1.5%
13 April 2016 2,454 11 View