Any one please explain this.
in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...
03 March 2024 9,306 1 View
For inderscience publication I need 4 expert in marketing domain. In the Step 5 of the submission process: Nomination of Four International Experts You are required to nominate four experts in...
05 February 2024 7,049 3 View
we were all know for composting process, but due to fowl smell and other not happening problems, people avoid home composting which are required for save of environment? its a very simple and...
19 January 2024 1,474 3 View
Dear fellow researchers, I am trying to simulate the temperature variation during a laser sintering of some metal powder. Material properties (density and conductivity) depend on temperature. I...
03 October 2023 1,771 2 View
Langyage problem
30 September 2023 9,145 1 View
I wish to isolate SP-D proteins and I was not sure why the protocol mentions the use of MnCl2 as an elution buffer to elute the SP-D protein from the maltose agarose beads column. Any help would...
25 June 2023 5,650 2 View
Dear amazing members, I have a doubt. If I have three adjacent planes with different boundary conditions, in a 3D domain, Dirichlet (fixed temperature) on one plane, Neumann fixed flux on...
21 June 2023 4,439 2 View
Hi there, i have been trying to achieve consistent smooth surface in FRP pipes lately . i was able to figure out how to negate dents, craters, crack holes ( occur due to SM Matt...
27 November 2022 691 0 View
Types of desiccators to be used for commercial multiplication of EPF. Vacuum desiccators Or chemical desiccators using nitrogen gas as a desiccant.
06 October 2022 9,537 0 View
I have been working with 40uM mouse brain sections mounted on triple coated chrome alum gelatin coverglass for multiplex imaging. By Round 2 I see these fluorescent specs/precipitate/debris all...
17 July 2022 9,206 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
