Any one please explain this.
we have problem in our field experiment of safed musali (Chlorophytum borivilianum ) cultivation. there is attack of termite in the soil which damage the roots in plant. we approached for organic...
02 September 2019 8,488 1 View
For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....
27 January 2019 7,112 19 View
A figure like the given one.
29 August 2017 3,374 2 View
I am trying get results from the hfss after design information an antenna and wanted to find if my impedance is matched or not.at 50om .so how can I do that....thanks in advance
18 May 2017 948 5 View
Hello, I am trying to tie a Eulerian object to a Lagrangian body or surface in order to give the Eulerian material translational and angular velocities using ABAQUS/CAE. In the ABAQUS...
07 March 2017 7,230 6 View
There are many softwares available, but can I know how to analyze XPS using origin or qtiplot etc plotting software?
01 February 2017 2,486 3 View
I have created multiple mutations of a single protein and I need to carry out energy minimization of those mutants to check stability. Please suggest some program or software or script.
08 November 2016 417 3 View
No ionization integrals possible at this bias point. Possibly applied voltage is less than DELTAV in field line calculation or the electrode does not contact any semiconductor material.
20 October 2016 8,761 2 View
Hello Everyone ! I'm doing analysis of a welding process using Coupled Eulerian-Lagrangian approach in ABAQUS/explicit. I have created a model (two layers of workpiece, one eulerian part, and a...
21 June 2016 9,323 10 View
Hello, my model of friction welding process is failing due to excessive distortion, although I've specified ALE adaptive mesh for the concerned domain. I need help in giving adaptive meshing...
21 May 2016 3,747 3 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
